+ DNA isolation from:

• biopsy samples (fresh frozen or paraffin embedded)
• fresh frozen tumor tissue (and corresponding normal tissue)
• peripheral blood
• mono nuclear cells (MNC)
• paraffin embedded tissue (stored for 10ys and more)
• cultivated cells/ cell lines

+ RNA isolation from:

• fresh frozen tumor tissue (and corresponding normal tissue)
• mono nuclear cells

+ Polymerase chain reaction (PCR)

+ Mutant allele specific amplification  (MASA)

+ Reverse Transcription (RT)

+ Real-time Quantitative - PCR

   (ABI Prism 5700 Sequence Detection System)

+ Restriction fragment length polymorphism -  Analysis (RFLP)

+ Blotting:

• Western Blot
• Northern Blot
• Southern Blot
  

+ Mutation scanning by sequencing:  

• radiolabeled (33P) terminator cycle sequencing
• ABI Prism ® 310 Genetic Analyzer

+ Immunohistochemistry (in cooperation with pathology) of:

• -p53
• -HER2/NEU
• BCL2
• MDM2 

+ Apoptosis detection:

• TUNEL assay